Article
Genetic typing of the DQA1*4 alleles by restriction enzyme digestion of the PCR product obtained with the DQ Alpha Amplitype kit.
Journal of forensic sciences - 1 Mar 1994
Salazar M, Williamson J, Bing D H
Abstract excerpt
The three DQ Alpha 4 alleles, 4.1 (0501), 4.2 (0401), and 4.3 (0601) cannot be distinguished with the reverse dot blot DQ Alpha Amplitype Kit. A previous report (Yunis, I. et al., Tissue Antigens, Vol. 39, 1992, pp. 182-186) indicated that the typing of these three alleles can be accomplished by endonuclease digestion of the PCR product that is produced following amplification with the DQ Alpha primers with Fok I...
Topics
- Alleles
- Base Sequence
- DNA
- Evaluation Studies as Topic
- Forensic Medicine
- Gene Frequency
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction