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Article

Mutational analysis of N-glycosylation recognition sites on the biochemical properties of Aspergillus kawachii alpha-L-arabinofuranosidase 54.

Biochimica et biophysica acta - 1 Sept 2006

Koseki Takuya, Miwa Yozo, Mese Yuichiro, Miyanaga Akimasa, Fushinobu Shinya, Wakagi Takayoshi, Shoun Hirofumi, Matsuzawa Hiroshi, Hashizume Katsumi

Abstract excerpt

A role for N-linked oligosaccharides on the biochemical properties of recombinant alpha-l-arabinofuranosidase 54 (AkAbf54) defined in glycoside hydrolase family 54 from Aspergillus kawachii expressed in Pichia pastoris was analyzed by site-directed mutagenesis. Two N-linked glycosylation motifs (Asn(83)-Thr-Thr and Asn(202)-Ser-Thr) were found in the AkAbf54 sequence. AkAbf54 comprises two domains, a catalytic...

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PMID: 16784813
DOI: 10.1016/j.bbagen.2006.04.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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