Article
Development of 198 novel EST-derived microsatellites in Eucalyptus (Myrtaceae).
American journal of botany - 1 Apr 2012
He Xudong, Wang Yu, Li Fagen, Weng Qijie, Li Mei, Xu Li-an, Shi Jisen, Gan Siming
Abstract excerpt
PREMISE OF THE STUDY: Expressed sequence tag (EST)-derived microsatellites were identified in Eucalyptus through screening the GenBank database. The loci were sequence-verified and explored for polymorphism among 20 genotypes. METHODS AND RESULTS: In total, 198 novel microsatellites were developed from 8262 unigenes, with the identity of 73.6-100% to the original sequences and presence of the expected repeat...
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