Article
Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships.
The Journal of biological chemistry - 11 Aug 2006
Beloqui Ana, Pita Marcos, Polaina Julio, Martínez-Arias Arturo, Golyshina Olga V, Zumárraga Miren, Yakimov Michail M, García-Arellano Humberto, Alcalde Miguel, Fernández Víctor M, Elborough Kieran, Andreu José M, Ballesteros Antonio, Plou Francisco J, Timmis Kenneth N, Ferrer Manuel, Golyshin Peter N
Abstract excerpt
RL5, a gene coding for a novel polyphenol oxidase, was identified through activity screening of a metagenome expression library from bovine rumen microflora. Characterization of the recombinant protein produced in Escherichia coli revealed a multipotent capacity to oxidize a wide range of substrates (syringaldazine > 2,6-dimethoxyphenol > veratryl alcohol > guaiacol > tetramethylbenzidine > 4-methoxybenzyl...
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