Expression and activity of chimeric molecules between human UDP-galactose transporter and CMP-sialic acid transporter.

doi:10.1093/oxfordjournals.jbchem.a022538

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Article

Expression and activity of chimeric molecules between human UDP-galactose transporter and CMP-sialic acid transporter.

Journal of biochemistry - 1 Nov 1999

Aoki K, Sun-Wada G H, Segawa H, Yoshioka S, Ishida N, Kawakita M

Abstract excerpt

Human UDP-galactose transporter (hUGT1) and CMP-sialic acid transporter (hCST) are related Golgi proteins with eight putative transmembrane helices predicted by computer analysis. We constructed chimeric molecules in which segments of various lengths from the C- or N-terminus of hUGT1 were replaced by corresponding portions of hCST. The chimeras were transiently expressed in UGT-deficient mutant Lec8 cells, and...

Topics

Animals
Base Sequence
Biological Transport, Active
CHO Cells
Carrier Proteins
Cricetinae
Cytidine Monophosphate N-Acetylneuraminic Acid
DNA Primers
DNA, Recombinant
Endoplasmic Reticulum
Gene Expression

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Golgi Apparatus

Humans

In Vitro Techniques

Membrane Proteins

Monosaccharide Transport Proteins

Mutation

Nucleotide Transport Proteins

Source

Open in PubMed

Identifiers

PMID: 10544289
DOI: 10.1093/oxfordjournals.jbchem.a022538

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