Article
Expression and activity of chimeric molecules between human UDP-galactose transporter and CMP-sialic acid transporter.
Journal of biochemistry - 1 Nov 1999
Aoki K, Sun-Wada G H, Segawa H, Yoshioka S, Ishida N, Kawakita M
Abstract excerpt
Human UDP-galactose transporter (hUGT1) and CMP-sialic acid transporter (hCST) are related Golgi proteins with eight putative transmembrane helices predicted by computer analysis. We constructed chimeric molecules in which segments of various lengths from the C- or N-terminus of hUGT1 were replaced by corresponding portions of hCST. The chimeras were transiently expressed in UGT-deficient mutant Lec8 cells, and...
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