Article
Allelic drop-out may occur with a primer binding site polymorphism for the commonly used RFLP assay for the -1131T>C polymorphism of the Apolipoprotein AV gene.
Lipids in health and disease - 2 May 2006
Ward Kirsten J, Ellard Sian, Yajnik Chittaranjan S, Frayling Timothy M, Hattersley Andrew T, Venigalla Prathyusha N S, Chandak Giriraj R
Abstract excerpt
Apolipoprotein AV (ApoAV) gene variant, -1131T>C, is associated with increased triglyceride concentrations in all ethnic groups studied. An MseI based RFLP analysis is the most commonly used method for genotyping this SNP. We genotyped a large cohort comprising 1185 Asian Indians and 173 UK Caucasians for -1131T>C using an ARMS-PCR based tetra-primer method. For quality control, we re-genotyped approximately 10%...
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