Article
Molecular cloning of the ViaB region of Salmonella typhi.
FEMS microbiology letters - 15 Dec 1991
Hashimoto Y, Ezaki T, Li N, Yamamoto H
Abstract excerpt
The ViaB region required for Vi antigen production in Salmonella typhi was cloned. The plasmid pGBM124 containing a 14-kb S. typhi chromosomal DNA fragment conferred the ability to produce Vi antigen on Escherichia coli HB101 and ViaB-deleted S. typhi GIFU10007-3. Tn5 insertion analysis showed that the 14-kb DNA was split into three regions. Region 1 and region 2 are involved in the biosynthesis of Vi...
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