Article
Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene.
Diagnostic microbiology and infectious disease - 1 Aug 2006
Delmas Julien, Breysse Franck, Devulder Gregory, Flandrois Jean-Pierre, Chomarat Monique
Abstract excerpt
Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed...
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