Article
Shortcut detection of the vanB gene cluster in enterococci by a duplex real-time PCR assay.
Pathology - 1 Aug 2006
Adams D N
Abstract excerpt
AIM: To develop a robust, simple and rapid method for detection of vanB in enterococci. METHODS: A real-time duplex PCR assay for the simultaneous detection of Enterococcus faecium and vanB resistance genotype in enterococci was developed in conjunction with a simple method for DNA extraction. The assay was tested on 130 fresh plate cultures of clinical isolates of enterococci and other Gram-positive bacteria....
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