Mutant bias in nonlethal selections results from selective recovery of mutants.

Abstract excerpt

We have characterized a nonlethal selection for mutations that allow Escherichia coli to grow on large maltodextrins (Dex+) in the absence of the lamB encoded maltoporin LamB. These Dex+ mutations occur before and after imposition of the selection and the selection does not result in a general increase in mutagenesis. The recovered Dex+ mutations are almost exclusively mutations that alter the ompF gene that...

Topics

• Bacterial Outer Membrane Proteins
• Escherichia coli
• Gene Expression
• Mutation
• Polysaccharides
• Porins
• Selection, Genetic