Article
An Escherichia coli rpoB mutation that inhibits transcription of catabolite-sensitive operons.
Journal of molecular biology - 20 Nov 1991
Rockwell P, Gottesman M E
Abstract excerpt
The Escherichia coli rpoB636 mutant is defective in the transcription of lac and other catabolite-sensitive operons. The lac promoter variant, UV5, which is independent of cyclic AMP and the cyclic AMP receptor protein, CRP, was also defective in rpoB636 mutants. The activity of the lac UV5 promoter was restored to wild-type levels by deletion of cya (adenylate cyclase) or crp. Cyclic AMP and CRP apparently act...
Topics
- Bacteriophage lambda
- Cyclic AMP
- DNA Mutational Analysis
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Mutation
- Promoter Regions, Genetic
- Receptors, Cyclic AMP
- Structure-Activity Relationship