Use of Real-Time Quantitative Polymerase Chain Reaction to Monitor the Evolution of Brucella melitensis DNA Load During Therapy and Post-Therapy Follow-Up in Patients with Brucellosis

Abstract excerpt

BACKGROUND: We performed quantitative real-time polymerase chain reaction (Q-PCR) to monitor the evolution of Brucella melitensis DNA load from initial diagnosis through post-therapy follow-up in patients with brucellosis. METHODS: On the basis of real-time fluorometric quantification of PCR products, we used the ultra-rapid LightCycler system (Roche Diagnostics). We collected 180 peripheral blood samples from 18...

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