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Article

Real time polymerase chain reaction: a new powerful tool for the diagnosis of neurobrucellosis

18 Jun 2005

Abstract excerpt

BACKGROUND/METHODS: We compared the diagnostic yield of a real time polymerase chain reaction (PCR) assay in cerebrospinal fluid (CSF) samples with conventional microbiological techniques for the diagnosis of neurobrucellosis. Following amplification of a 223 bp sequence specific for Brucella genus, melting curve analysis was performed to verify the specificity of the PCR products. RESULTS: All six patients with...

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PMID: 15965220
DOI: 10.1136/jnnp.2004.049411

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