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Article

Real-time PCR for simultaneous detection and quantification of Borrelia burgdorferi in field-collected Ixodes scapularis ticks from the Northeastern United States.

Applied and environmental microbiology - 1 Aug 2003

Wang Guiqing, Liveris Dionysios, Brei Brandon, Wu Hongyan, Falco Richard C, Fish Durland, Schwartz Ira

Abstract excerpt

The density of spirochetes in field-collected or experimentally infected ticks is estimated mainly by assays based on microscopy. In this study, a real-time quantitative PCR (qPCR) protocol targeting the Borrelia burgdorferi-specific recA gene was adapted for use with a Lightcycler for rapid dete...

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Identifiers

PMID: 12902243
DOI: 10.1128/AEM.69.8.4561-4565.2003
PMCID: PMC169074

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-12902243-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

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