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Article

Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks

2010-08-01

Abstract excerpt

A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for Borrelia detection as compared to stan...

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Identifiers and source

Literature Corpus work
94be3a2c-7b19-557b-b23e-848f7bb17830
DOI
10.1590/s0102-09352010000400015
Open publication

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Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticksDOI 10.1590/s0102-09352010000400015
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