Expression of mouse mammary tumor virus glycoprotein truncations defines roles for the transmembrane domain and ectodomain hydrophobic region in constitutive exocytic trafficking and proteolytic processing.

Abstract excerpt

A mutational analysis was used to identify structural domains that are important for exocytic transport and proteolytic cleavage of the mouse mammary tumor virus (MMTV) glycoprotein, which is expressed as a multidomain polyprotein. Rat HTC hepatoma cells were transfected with the MMTV glycoprotein gene driven by the constitutive Rous sarcoma virus promoter, with mutant genes encoding a series of polypeptide...

Topics

• Blotting, Northern
• Electrophoresis, Polyacrylamide Gel
• Exocytosis
• Gene Expression
• Glycoproteins
• Hydrolysis
• Mammary Tumor Virus, Mouse
• Mutation
• Plasmids
• Proviruses