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Novel Technique of Quantitative Nested Real-Time PCR Assay for Mycobacterium tuberculosis DNA

1 Mar 2006

Abstract excerpt

The diagnosis of tuberculous meningitis (TBM) remains a complex issue because the most widely used conventional diagnostic tools, such as culture and PCR assay for cerebrospinal fluid (CSF) samples, are unable to rapidly detect Mycobacterium tuberculosis with sufficient sensitivity in the acute phase of TBM. Based on TaqMan PCR, we designed a novel technique consisting of an internally controlled quantitative...

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PMID: 16517891
DOI: 10.1128/jcm.44.3.1029-1039.2006

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