Article
A yeast two-hybrid knockout strain to explore thioredoxin-interacting proteins in vivo.
Proceedings of the National Academy of Sciences of the United States of America - 15 Nov 2005
Vignols Florence, Bréhélin Claire, Surdin-Kerjan Yolande, Thomas Dominique, Meyer Yves
Abstract excerpt
All organisms contain thioredoxin (TRX), a regulatory thiol:disulfide protein that reduces disulfide bonds in target proteins. Unlike animals and yeast, plants contain numerous TRXs for which no function has been assigned in vivo. Recent in vitro proteomic approaches have opened the way to the identification of >100 TRX putative targets, but of which none of the numerous plant TRXs can be specifically associated....
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