Article
A high throughput method for rapid screening of chitosanase-producing fungal strain under acidic conditions.
World journal of microbiology & biotechnology - 1 Nov 2016
Ding Su, Chen Gui-Guang, Liang Zhi-Qun, Zeng Wei, Cao Mu-Ming, Chen Guo-Pin, Xie Shu-Yu, Li Wei
Abstract excerpt
A novel high-throughput method was established for rapid screening of a large numbers of Aspergillus fumigatus (A. fumigatus) mutants with high chitosanase production under acidic culture condition by exploiting the fact that iodine can be used as the indicator to stain chitosan but is ineffective for chitooligosaccharides. The mutant population was generated by irradiating A. fumigatus CICC 2434 with Co(60)-γ...
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