Article
Transfer of Tn916 and Tn916 delta E into Clostridium difficile: demonstration of a hot-spot for these elements in the C. difficile genome.
FEMS microbiology letters - 15 Apr 1991
Mullany P, Wilks M, Tabaqchali S
Abstract excerpt
The conjugative transposon Tn916 and a derivative Tn916 delta E was transferred from Bacillus subtilis into Clostridium difficile CD37 by filter mating. All the C. difficile transconjugants appeared to contain one copy of the transposon integrated into the same position in the genome. Transpositi...
Topics
- Bacillus subtilis
- Clostridioides difficile
- Conjugation, Genetic
- DNA Transposable Elements
- Genes, Bacterial
- Mutation
- Plasmids
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Tetracycline Resistance
- Transformation, Genetic