Article
Bacterial DNA supercoiling and [ATP]/[ADP] ratio: changes associated with salt shock.
Journal of bacteriology - 1 Jun 1991
Hsieh L S, Rouviere-Yaniv J, Drlica K
Abstract excerpt
When Escherichia coli K-12 was shifted from a medium lacking salt to one containing 0.5 M NaCl, both the [ATP]/[ADP] ratio and negative supercoiling of plasmid DNA increased within a few minutes. After about 10 min both declined, eventually reaching a level slightly above that observed with cells growing exponentially in the absence of salt. Since in vitro the [ATP]/[ADP] ratio influences the level of...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphate
- Aminocoumarins
- Coumarins
- DNA Topoisomerases, Type II
- DNA, Bacterial
- DNA, Superhelical
- Escherichia coli
- Mutation
- Plasmids
- Sodium Chloride