Genetic variation and cross-reactivity of Clostridium septicum alpha-toxin.

doi:10.1016/j.vetmic.2005.10.039

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Article

Genetic variation and cross-reactivity of Clostridium septicum alpha-toxin.

Veterinary microbiology - 16 Apr 2006

Amimoto Katsuhiko, Sasaki Yoshimasa, Fukuyama Shin-ichi, Tamura Yutaka

Abstract excerpt

Clostridium septicum alpha-toxin genes were sequenced with the polymerase chain reaction (PCR) products amplified from DNAs of 25 C. septicum strains, and were classified into 10 patterns. Alpha-toxins were purified from the culture supernatant of four C. septicum strains (strains No. 44, Kagoshima 8, Mie and Tokachi) which were specially chosen from patterns of the deduced amino acid sequences. The molecular...

Topics

Amino Acid Sequence
Animals
Antitoxins
Bacterial Toxins
Base Sequence
Cattle
Chlorocebus aethiops
Clostridium septicum
Cross Reactions
DNA Primers
Genetic Variation
Lethal Dose 50
Male
Mice
Molecular Sequence Data
Neutralization Tests
Phylogeny
Polymerase Chain Reaction

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PMID: 16337096
DOI: 10.1016/j.vetmic.2005.10.039

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