Article
Two-dimensional fluorescence difference gel electrophoretic analysis of Streptococcus mutans biofilms.
Journal of proteome research - 1 Jan 2000
Rathsam Catherine, Eaton Ruth E, Simpson Christine L, Browne Gina V, Valova Valentina A, Harty Derek W S, Jacques Nicholas A
Abstract excerpt
Compared with traditional two-dimensional (2D) proteome analysis of Streptococcus mutans grown as a biofilm from a planktonic culture at steady state (Rathsam et al., Microbiol. 2005, 151, 1823-1837), the use of 2D fluorescence difference gel electrophoresis (DIGE) led to a 3-fold increase in the number of identified protein spots that were significantly altered in their level of expression (P < 0.050). Of the 73...
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