Article
Conformational Heterogeneity and the Affinity of Substrate Molecular Recognition by Cytochrome P450cam.
Biochemistry - 27 Jun 2017
Basom Edward J, Manifold Bryce A, Thielges Megan C
Abstract excerpt
The broad and variable substrate specificity of cytochrome P450 enzymes makes them a model system for studying the determinants of protein molecular recognition. The archetypal cytochrome P450cam (P450cam) is a relatively specific P450, a feature once attributed to the high rigidity of its active site. However, increasingly studies have provided evidence of the importance of conformational changes to P450cam...
Topics
- Camphor 5-Monooxygenase
- Catalytic Domain
- Crystallography, X-Ray
- Humans
- Hydrogen Bonding
- Models, Molecular
- Mutation
- Protein Binding
- Protein Conformation
- Substrate Specificity