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Article

Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases.

Journal of general microbiology - 1 Oct 1993

Kok R G, Christoffels V M, Vosman B, Hellingwerf K J

Abstract excerpt

Acinetobacter calcoaceticus BD413, when grown in batch culture in nutrient broth, produces both extracellular lipase activity and cell-bound esterase activity during and after the transition between exponential growth and the stationary phase. From a library of A. calcoaceticus DNA in Escherichia coli, plasmids were isolated that enabled E. coli to grow on media with tributyrin as the sole carbon source. Assays...

Topics

Acinetobacter calcoaceticus
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Escherichia coli
Esterases
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genetic Vectors
Lac Operon

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Lipolysis

Molecular Sequence Data

Mutation

Plasmids

Recombinant Fusion Proteins

Restriction Mapping

Sequence Analysis, DNA

Sequence Deletion

Source

Identifiers

PMID: 8254303
DOI: 10.1099/00221287-139-10-2329

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8254303-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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