Article
Cloning and characterization of a chromosomal DNA region required for growth on 2,4,5-T by Pseudomonas cepacia AC1100.
Gene - 1 Apr 1991
Haugland R A, Sangodkar U M, Sferra P R, Chakrabarty A M
Abstract excerpt
A series of spontaneous 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) nonmetabolizing mutants of Pseudomonas cepacia AC1100 were characterized to be defective in either 2,4,5-T uptake or conversion of this compound to 2,4,5-trichlorophenol (2,4,5-TCP). Two of these mutants, RHC22 and RHC23, were complemented for growth on 2,4,5-T using an AC1100 genomic library constructed in the cosmid vector pCP13. Recombinant...
Topics
- 2,4,5-Trichlorophenoxyacetic Acid
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Mixed Function Oxygenases
- Mutation
- Phenotype
- Plasmids