Article
Testing of a whole genome PCR scanning approach to identify genomic variability in four different species of lactic acid bacteria.
Research in microbiology - 1 May 2006
Ben Zakour Nouri, Grimaldi Christine, Gautier Michel, Langella Philippe, Azevedo Vasco, Maguin Emmanuelle, Le Loir Yves
Abstract excerpt
Genomes can be markedly heterogeneous in conspecific bacterial strains. Genome sequences can be used to analyze genome plasticity via a PCR(2) (plasticity of chromosome revealed by PCR) approach. Small-sized chromosomes can indeed be fully amplified by long-range PCR with a set of primers designed using a reference strain and then applied to several other strains. Analysis of the resulting patterns can reveal...
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