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Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene

1 Jul 1992

Abstract excerpt

Synthetic oligonucleotide primers of 21 and 24 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence of the nuc gene, which encodes the thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from lysed S. aureus cells or isolated DNA. The PCR product was detected by agarose gel electrophoresis or Southern blot analysis by using a...

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PMID: 1629319
DOI: 10.1128/jcm.30.7.1654-1660.1992

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