Multiplex polymerase chain reaction for detection of genes for<i>Staphylococcus aureus</i>thermonuclease and methicillin resistance and correlation with oxacillin resistance

Abstract excerpt

A multiplex polymerase chain reaction (mPCR) was used for simultaneous amplification of the staphylococcal nuc gene, encoding the thermostable nuclease (TNase), and the mecA gene, encoding the penicillin-binding protein 2a which is associated with staphylococcal methicillin resistance. A total of 219 staphylococcal strains were tested and the mPCR data were compared with coagulase production and in vitro...

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