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Development of a PCR Assay for Identification of Staphylococci at Genus and Species Levels

1 Jul 2001

Abstract excerpt

We have developed a PCR-based assay which allows the detection of staphylococci at the genus level by targeting the tuf gene, which encodes the elongation factor Tu. Degenerate PCR primers derived from consensus regions of several tuf genes were used to amplify a target region of 884 bp from 11 representative staphylococcal species. Subsequently, the entire nucleotide sequence of these amplicons was determined....

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PMID: 11427566
DOI: 10.1128/jcm.39.7.2541-2547.2001

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