Article
Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction.
Journal of clinical microbiology - 1 Mar 1991
Johnson W M, Tyler S D, Ewan E P, Ashton F E, Pollard D R, Rozee K R
Abstract excerpt
Eight pairs of synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to detect genes for staphylococcal enterotoxins A to E, exfoliative toxins A and B, and toxic shock syndrome toxin 1 in Staphylococcus aureus strains isolated from clinical specimens and contaminated foods. Primers were targeted to internal regions of the toxin genes, and amplification fragments were detected...
Topics
- Bacterial Toxins
- Base Sequence
- DNA, Bacterial
- Enterotoxins
- Exfoliatins
- Genes, Bacterial
- Genotype
- Humans
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
