Article
Targeted suppression of E-cadherin gene expression in bovine preimplantation embryo by RNA interference technology using double-stranded RNA.
Molecular reproduction and development - 1 Feb 2006
Nganvongpanit Korakot, Müller Heike, Rings Franca, Gilles Markus, Jennen Danyel, Hölker Michael, Tholen Ernst, Schellander Karl, Tesfaye Dawit
Abstract excerpt
RNA interference (RNAi) has become acknowledged as an effective and useful tool to study gene function in diverse groups of cells. We aimed to suppress the expression of the E-cadherin gene during in vitro development of bovine preimplantation embryos using RNAi approach. In this experiment the effect of microinjection of E-cadherin and Oct-4 (as control) double-stranded (ds) RNA on the mRNA and protein...
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