Article
Analysis of actinomycin D treated cattle oocytes and their use for somatic cell nuclear transfer.
Animal reproduction science - 1 Dec 2008
Moura Marcelo Tigre, de Sousa Regivaldo Vieira, de Oliveira Leme Ligiane, Rumpf Rodolfo
Abstract excerpt
The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, for oocyte chemical enucleation. Cattle oocytes matured in vitro were treated with actinomycin D according to the following treatments: T1, control; T2=1.0 microg/ml for 16 h; T3=1.0 microg/ml for 14 h; T4=2.5 microg/ml for 14 h; T5=5.0 microg/ml for 14 h. The oocytes were denuded and activated during 24-26 h of...
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