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Article

Antagonism, non-native interactions and non-two-state folding in S6 revealed by double-mutant cycle analysis.

Protein engineering, design & selection : PEDS - 1 Nov 2005

Otzen Daniel

Abstract excerpt

When folding to the native state N in the presence of salt, the apparent two-state folder S6 transiently forms a transient off-pathway state C with substantial secondary and tertiary structure. Fifteen double mutant cycles were analysed to compare side-chain interaction energies DeltaDeltaG(int) in C, N and TS (the transition state between N and the denatured state). The kinetic signatures of these destabilizing...

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PMID: 16230299
DOI: 10.1093/protein/gzi063

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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