Article
A combination of decreased NRTI incorporation and decreased excision determines the resistance profile of HIV-1 K65R RT.
AIDS (London, England) - 4 Nov 2005
White Kirsten L, Margot Nicolas A, Ly John K, Chen James M, Ray Adrian S, Pavelko Martina, Wang Ruth, McDermott Martin, Swaminathan S, Miller Michael D
Abstract excerpt
OBJECTIVE: To determine the mechanisms of resistance of K65R mutant reverse transcriptase (RT) to the currently approved nucleoside and nucleotide RT inhibitors (NRTI). METHODS: Susceptibilities of K65R mutant HIV-1 to NRTI were determined in cell culture. The Ki/Km values were measured to determine the relative binding or incorporation of the NRTI, and ATP-mediated excision of incorporated NRTI was measured to...
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