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Development of an F57 Sequence-Based Real-Time PCR Assay for Detection ofMycobacterium aviumsubsp.paratuberculosisin Milk

1 Oct 2005

Abstract excerpt

A light cycler-based real-time PCR (LC-PCR) assay that amplifies the F57 sequence of Mycobacterium avium subsp. paratuberculosis was developed. This assay also includes an internal amplification control template to monitor the amplification conditions in each reaction. The targeted F57 sequence element is unique for M.avium subsp. paratuberculosis and is not known to exist in any other bacterial species. The...

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PMID: 16204510
DOI: 10.1128/aem.71.10.5957-5968.2005

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