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New Triplex Real-Time PCR Assay for Detection of<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>in Bovine Feces

8 Mar 2008

Abstract excerpt

In the present study, a robust TaqMan real-time PCR amplifying the F57 and the ISMav2 sequences of Mycobacterium avium subsp. paratuberculosis from bovine fecal samples was developed and validated. The validation was based on the recommendations of International Organization for Standardization protocols for PCR and real-time PCR methods. For specificity testing, 205 bacterial strains were selected, including 105...

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PMID: 18326682
DOI: 10.1128/aem.02534-07

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