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Duplex PCR for Differential Identification ofMycobacterium bovis,M. avium, andM. aviumsubsp.paratuberculosisin Formalin- Fixed Paraffin-Embedded Tissues from Cattle

1 Aug 2000

Abstract excerpt

We previously isolated and sequenced two genomic segments of Mycobacterium avium subsp. paratuberculosis, namely, f57, a species-specific sequence, and the p34 gene, coding for a 34-kDa antigenic protein. Comparison of sequences upstream of the p34 open reading frame (us-p34) from M. avium subsp. paratuberculosis and M. tuberculosis showed a 79-base deletion in M. tuberculosis. Sequence analysis of the p34 genes...

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PMID: 10921976
DOI: 10.1128/jcm.38.8.3048-3054.2000

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