Development of Multiplex Polymerase Chain Reaction Assays for Detecting Enterotoxigenic Escherichia Coli and their Application to Field Isolates from Piglets with Diarrhea

Abstract excerpt

Fimbriae and enterotoxins are major virulence factors associated with enterotoxigenic Escherichia coli (ETEC). In this study, 3 sets of multiplex polymerase chain reaction (mPCR) assays targeting fimbriae, enterotoxins, and other adherence factors were developed for detecting ETEC. A total number of 188 E. coli field isolates were examined, and percentages of E. coli strains carrying each virulence factors were...

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