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Article

Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus.

Journal of virology - 1 Jul 1992

Lee H Y, Krell P J

Abstract excerpt

We have generated defective genomes of Autographa californica nuclear polyhedrosis virus (AcNPV) by serial, undiluted passage in IPLB-SF-21 cell culture in an attempt to identify potential cis-acting sequences important for AcNPV DNA replication. Viral DNA isolated from some of the 81 serial passages was analyzed by pulsed-field gel electrophoresis, restriction endonuclease analysis, and Southern blot...

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Identifiers

PMID: 1602548
DOI: 10.1128/JVI.66.7.4339-4347.1992
PMCID: PMC241240

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