Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus.

doi:10.1128/JVI.66.7.4339-4347.1992

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Article

Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus.

Journal of virology - 1 Jul 1992

Lee H Y, Krell P J

Abstract excerpt

We have generated defective genomes of Autographa californica nuclear polyhedrosis virus (AcNPV) by serial, undiluted passage in IPLB-SF-21 cell culture in an attempt to identify potential cis-acting sequences important for AcNPV DNA replication. Viral DNA isolated from some of the 81 serial pass...

Topics

Animals
Baculoviridae
Blotting, Southern
Cell Line
DNA, Viral
Gene Amplification
Genome, Viral
Phenotype
Restriction Mapping
Serial Passage

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