Article
Functional characterization of Autographa californica multiple nucleopolyhedrovirus ORF43 and phenotypic changes of ORF43-knockout mutant.
Journal of microbiology (Seoul, Korea) - 1 Aug 2013
Tao Xue Ying, Choi Jae Young, Wang Yong, Roh Jong Yul, Lee Joo Hyun, Liu Qin, Park Jong Bin, Kim Jae Su, Kim Woojin, Je Yeon Ho
Abstract excerpt
ORF43 (ac43) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene of unknown function. To investigate the role of ac43 in the baculovirus lifecycle, we constructed an ac43-deleted mutant AcMNPV, Ac43KO. After transfection into Spodoptera frugiperda cells, Ac43KO produced polyhedra much larger in size than those of wild-type AcMNPV. Interestingly, some of the...
Topics
- Animals
- Cell Line
- Gene Deletion
- Gene Expression Regulation, Viral
- Gene Knockout Techniques
- Gene Order
- Inclusion Bodies, Viral
- Moths
- Mutation
- Nucleopolyhedroviruses
- Phenotype
- Transcription, Genetic
- Viral Proteins