Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication.

doi:10.1128/JVI.66.9.5525-5533.1992

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication.

Journal of virology - 1 Sept 1992

Hershberger P A, Dickson J A, Friesen P D

Abstract excerpt

The gene encoding the 35-kDa protein (35k gene) located within the EcoRI-S genome fragment of Autographa californica nuclear polyhedrosis virus (AcMNPV) is transcribed early in infection. To examine its function(s) with respect to virus multiplication, we introduced specific mutations of this early gene into the AcMNPV genome. In Spodoptera frugiperda (SF21) culture, deletion of the 35K gene reduced yields of...

Topics

Amino Acid Sequence
Animals
Baculoviridae
Cell Line
DNA, Viral
Gene Expression Regulation, Viral
Genetic Complementation Test
Molecular Sequence Data
Moths
Mutagenesis
Mutagenesis, Site-Directed

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Reading Frames

Recombinant Fusion Proteins

Transcription, Genetic

Viral Proteins

Virus Replication

Source

Open in PubMed Open full text

Identifiers

PMID: 1501287
DOI: 10.1128/JVI.66.9.5525-5533.1992
PMCID: PMC289111

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1501287-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication.PMID 1501287Read ↗

Mutations in the Autographa californica multinucleocapsid nuclear polyhedrosis virus 25 kDa protein gene result in reduced virion occlusion, altered intranuclear envelopment and enhanced virus production.Semantic proximity Read ↗

Removal of transposon target sites from the Autographa californica multiple nucleopolyhedrovirus fp25k gene delays, but does not prevent, accumulation of the few polyhedra phenotype.Semantic proximity Read ↗

Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells.Semantic proximity Read ↗

Characterization of the DA26 gene in a hypervariable region of the Autographa californica nuclear polyhedrosis virus genome.Semantic proximity Read ↗

Abnormal formation of polyhedra resulting from a single mutation in the polyhedrin gene of Autographa californica multicapsid nucleopolyhedrovirus.Semantic proximity Read ↗

Detection and analysis of Autographa californica nuclear polyhedrosis virus mutants with defective interfering properties.Semantic proximity Read ↗

Cell-dependent production of polyhedra and virion occlusion of Autographa californica multiple nucleopolyhedrovirus fp25k mutants in vitro and in vivo.Semantic proximity Read ↗

The function of envelope protein P74 from Autographa californica multiple nucleopolyhedrovirus in primary infection to host.Semantic proximity Read ↗

Reduction of polyhedrin mRNA and protein expression levels in Sf9 and Hi5 cell lines, but not in Sf21 cells, infected with Autographa californica multiple nucleopolyhedrovirus fp25k mutants.Semantic proximity Read ↗

The structural and functional organization of the Autographa californica nuclear polyhedrosis virus genome.Semantic proximity Read ↗

Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus.Semantic proximity Read ↗

Biosynthesis and localization of the Autographa californica nuclear polyhedrosis virus 25K gene product.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.