Article
Development of methods for RNA interference in the sheep gastrointestinal parasite, Trichostrongylus colubriformis.
International journal for parasitology - 1 Aug 2005
Issa Z, Grant W N, Stasiuk S, Shoemaker C B
Abstract excerpt
The efficiency of RNA interference (RNAi) delivery to L1 through L3 stage worms of the sheep parasitic nematode Trichostrongylus colubriformis was investigated using several techniques. These were: (i) feeding of Escherichia coli expressing double stranded RNA (dsRNA); (ii) soaking of short interfering (synthetic) RNA oligonucleotides (siRNA) or in vitro transcribed dsRNA molecules; and (iii) electroporation of...
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