Article
The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis.
Genetics - 1 Nov 2005
Sanchez Humberto, Kidane Dawit, Reed Patricia, Curtis Fiona A, Cozar M Castillo, Graumann Peter L, Sharples Gary J, Alonso Juan C
Abstract excerpt
In models of Escherichia coli recombination and DNA repair, the RuvABC complex directs the branch migration and resolution of Holliday junction DNA. To probe the validity of the E. coli paradigm, we examined the impact of mutations in DeltaruvAB and DeltarecU (a ruvC functional analog) on DNA repair. Under standard transformation conditions we failed to construct DeltaruvAB DeltarecG, DeltarecU DeltaruvAB,...
Topics
- Bacillus subtilis
- Bacterial Proteins
- DNA Damage
- DNA Helicases
- DNA Repair
- DNA Restriction Enzymes
- DNA, Bacterial
- DNA, Cruciform
- Endodeoxyribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Genes, Reporter
- Holliday Junction Resolvases