Including mutations from conceptually translated expressed sequence tags into orthologous proteins improves the preliminary assignment of peptide mass fingerprints on non-model genomes.

Abstract excerpt

In order to improve protein assignment from peptide mass fingerprints (PMF) in species with incompletely sequenced genomes, the genus-specific mutations deduced from Expressed sequence tag (EST) sequences were included in the complete reading frames of orthologous proteins, resulting in a new searchable in silico protein database. Using this method in tests on four plant species, the MOWSE score of at least 20%...

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