Article
Identification of a crucial histidine involved in metal transport activity in the Arabidopsis cation/H+ exchanger CAX1.
The Journal of biological chemistry - 26 Aug 2005
Shigaki Toshiro, Barkla Bronwyn J, Miranda-Vergara Maria Cristina, Zhao Jian, Pantoja Omar, Hirschi Kendal D
Abstract excerpt
In plants, yeast, and bacteria, cation/H+ exchangers (CAXs) have been shown to translocate Ca2+ and other metal ions utilizing the H+ gradient. The best characterized of these related transporters is the plant vacuolar localized CAX1. We have used site-directed mutagenesis to assess the impact of altering the seven histidine residues to alanine within Arabidopsis CAX1. The mutants were expressed in a...
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