Article
Mutants of the Arabidopsis thaliana cation/H+ antiporter AtNHX1 conferring increased salt tolerance in yeast: the endosome/prevacuolar compartment is a target for salt toxicity.
The Journal of biological chemistry - 22 May 2009
Hernández Agustín, Jiang Xingyu, Cubero Beatriz, Nieto Pedro M, Bressan Ray A, Hasegawa Paul M, Pardo José M
Abstract excerpt
Mutants of the plant cation/H(+) antiporter AtNHX1 that confer greater halotolerance were generated by random mutagenesis and selected in yeast by phenotypic complementation. The amino acid substitutions that were selected were conservative and occurred in the second half of the membrane-associated N terminus. AtNHX1 complemented the lack of endogenous ScNHX1 in endosomal protein trafficking assays. Growth...
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