Article
Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase.
Applied and environmental microbiology - 1 Apr 1992
Gaskell J, Vanden Wymelenberg A, Stewart P, Cullen D
Abstract excerpt
A method to identify and differentiate allelic variants of the gene encoding lignin peroxidase isozyme H8 is presented. The strategy involves amplifying a variable region of the gene's carboxy terminus by use of the polymerase chain reaction and then probing with allele-specific oligonucleotides.
Topics
- Alleles
- Base Sequence
- Basidiomycota
- Blotting, Southern
- DNA, Fungal
- Genes, Fungal
- Molecular Sequence Data
- Peroxidases
- Polymerase Chain Reaction