Article
Establishment of genetic linkage by allele-specific polymerase chain reaction: application to the lignin peroxidase gene family of Phanerochaete chrysosporium.
Bio/technology (Nature Publishing Company) - 1 Dec 1994
Gaskell J, Stewart P, Kersten P J, Covert S F, Reiser J, Cullen D
Abstract excerpt
Determining linkage is problematic for genes lacking easily identifiable phenotypes and for organisms without well-defined genetic recombination systems. Phanerochaete chrysosporium with its lignin peroxidase (LiP) gene family typifies these difficulties. We describe an experimental approach whereby the segregation of specific alleles is directly monitored during sexual fruiting. The method establishes linkage...
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