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An Improved Polymerase Chain Reaction Assay to Detect Trypanosoma cruzi in Blood

1 May 1992

Abstract excerpt

Amplification by the polymerase chain reaction of Trypanosoma cruzi satellite DNA was used to enhance sensitivity in the detection of the parasite in blood, with the ultimate goal of improving diagnosis of the chronic phase of Chagas' disease. Two contiguous oligonucleotides were synthesized corresponding to the most conserved region of the 195-basepair repeated sequence and used as primers for the amplification...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 1599057
DOI: 10.4269/ajtmh.1992.46.616

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